Biolegend, 939401, Purified anti-eIF3F Antibody, 25μg

The eukaryotic translation initiation factor 3 (eIF3) is an 800 kD oligomeric complex comprised of 13 different subunits and plays an essential role in protein translation. The eIF3 complex directly links the 43S pre-initiation complex to eIF4F-bound mRNA. The subunit eIF3F has been shown to negatively regulate both cap-dependent and IRES-dependent translation and also interacts with mTOR and S6K1, and therefore also regulates mTORC1 and mTORC2 signaling. EIF3F mRNA is downregulated in multiple types of cancer. Overexpression of eIF3F protein leads to hypertrophy by altering protein synthesis via the mTORC1 pathway. Under energy starvation conditions, eIF3F is ubiquitinated by the MAFbx/atrogen-1 complex and targeted for proteasomal degradation, leading to muscle atrophy.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant human eIF3F protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in poor eIF3F staining. This clone failed to immunoprecipitate eIF3F from HeLa lysates.
RRID: AB_2876767 (BioLegend Cat. No. 939401) AB_2876767 (BioLegend Cat. No. 939402)
Structure: eIF3F is a 357 amino acid protein with a predicted molecular weight of 38 kD.
Distribution: Ubiquitously expressed/Cytoplasm
Function: Protein Translation
Biology Area: Cell Biology, Protein Synthesis
Antigen References: Docquier A, et al. 2019. J Physiol. 597:3107. Sanchez AM, et al. 2013. Int J Biochem Cell Biol. 45:2158. Wen F, et al. 2012. PLoS One. 7:e34194. Csibi A, et al. 2010. PLoS One. 5:e8994. Doldan A, et al. 2008. Mol Carcinog.47:235.
Gene ID: 8665
UniProt: View information about eIF3F on UniProt.org
Clone: W19170B
Regulatory Status: RUO
Other Names: Eukaryotic Translation Initiation Factor 3 Subunit F, Deubiquitinating Enzyme EIF3f, EIF3-Epsilon, EIF3 P47, MRT67
Isotype: Rat IgG2a, κ