Biolegend, 941004, PE anti-SAMHD1 Antibody, 100tests

SAMHD1 is a host defense factor involved in the innate immune response to viral infections, including HIV-1. Through its dNTPase activity, SAMHD1 disrupts viral genome replication by depleting available pools of deoxynucleotide triphosphates (dNTPs) for viral reverse transcription in dendritic and myeloid cells. In addition to its role in innate immune defense, SAMHD1 also plays an essential role in preserving genome stability through maintaining balanced dNTP pools. Independently of its dNTPase activity, SAMHD1 is required for resection of stalled, gapped, or reversed DNA replication forks by stimulating the exonuclease activity of MRE11. SAMHD1-deficient cells show increased dNTPs and genome instability, and an increased accumulation of G1-positive cells. These cells also accumulate single-strand DNA fragments in the cytosol, leading to the induction of pro-inflammatory type 1 interferons through the cGAS-STING pathway. Mutations in the SAMHD1 are found in many cancers, as well as the severe congenital inflammatory disease Aicardi-Goutieres syndrome.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human SAMHD1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone W19081C is not recommended for immunocytochemistry applications due to non-specific staining.
Additional Product Notes: Clone W19081C can be used with Cyto-Fast™ Fix/Perm Buffer or True-Nuclear™ Transcription Factor Buffer. However, True-Phos™ Perm Buffer is recommended for optimal staining results.
RRID: AB_3662343 (BioLegend Cat. No. 941003) AB_3662343 (BioLegend Cat. No. 941004)
Structure: SAMHD1 is a 626 amino acid protein with a predicted molecular weight of 72 kD.
Distribution: Ubiquitously expressed/Nucleus
Function: Innate immune response, DNA replication and repair
Cell Type: Dendritic cells, Monocytes
Biology Area: Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Innate Immunity
Molecular Family: Enzymes and Regulators
Antigen References: Laguette N, et al. 2011. Nature. 474:654.
Gene ID: 25939
UniProt: View information about SAMHD1 on UniProt.org
Clone: W19081C
Regulatory Status: RUO
Other Names: SAM and HD Domain Containing Deoxynucleoside Triphosphate, Triphosphohydrolase 1, Monocyte Protein 5, Aicardi-Goutieres Syndrome 5, Sterile Alpha Motif and Histidine Aspartate Domain-Containing Protein 1
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.