Biolegend, 942004, Ultra-LEAF™ Purified anti-mouse CD27 Antibody, 1mg

CD27 (TNFRSF7) is a member of the TNFR family. It is a type I membrane glycoprotein with a MW of 55 kD that forms a disulfide‑linked homodimer. The receptor possesses three cysteine rich repeats in the extracellular domain, the transmembrane region, and the intracellular domain. CD27 binds CD27L (CD70), another member of the TNF ligand family that forms a trimer; thus, it has been speculated that the CD27L binds to a trimeric structure composed by three dimeric CD27 molecules. The interaction between CD27 and its ligand, CD70, plays a role in providing co-stimulation for prolonged lymphocyte survival, enhanced T-cell proliferation, and memory-cell formation. T cells expressing CD27 and CD70 play a key role in regulating B-cell activation and immunoglobulin synthesis. Soluble CD27 (sCD27), the extracellular domain of membrane-bound CD27, can be released after lymphocyte activation by differential splicing of the receptor protein or shedding from the cell surface by metalloproteinases (MMPs). Soluble CD27 has been detected in serum, plasma, and urine samples from healthy individuals, and it has been described elevated sCD27 levels in serum of patients with systemic lupus erythematosus, viral infections, and lymphoid malignancies. The sCD27-pool in cancer patients can be significantly enlarged by immunotherapy. CD27 is recognized as a marker for memory B cells and is considered of diagnostic value in patients who have decreased numbers of switched memory B cells.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD27 (TNFRSF7)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.08 µg/mL recombinant mouse CD27L to 2 µg/mL immobilized recombinant mouse CD27 (TNFRSF7)-Fc. ND50 = 0.3 - 1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2890861 (BioLegend Cat. No. 942003) AB_2890861 (BioLegend Cat. No. 942004)
Structure: Homodimer
Distribution: Resting T and B cells, NK cells, hematopoietic progenitor cells
Function: Induces T-cell co-stimulation and B-cell activation, plasma cell differentiation
Interaction: Activated T and B cells, antigen-presenting cells (APC)
Ligand/Receptor: CD27L (CD70)
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, Plasma cells, T cells, Thymocytes
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, Immune Checkpoint Receptors, Soluble Receptors
Antigen References: Camerini D, et al. 1991. J Immunol. 147:3165-9. Gravestein LA, et al. 1993. Eur J Immunol. 23:943-50. Prasad KV, et al. 1997. Proc Natl Acad Sci. 94:6346-51. Kato K, et al. 2007. Exp Hematol. 35:434-42. van Montfrans J M, et al. 2012. J Allergy Clin Immunol. 129:787-793. Goto N, et al. 2012. Leuk Lymphoma. 53:1494-500. Huang J, et al. 2013. J Immunol. 190:6250-8.
Gene ID: 21940
UniProt: View information about CD27 on UniProt.org
Clone: A18209D
Regulatory Status: RUO
Other Names: CD27 antigen, CD antigen 27, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, TNFRSF7T-cell activation antigen CD27
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.