Biolegend, 942201, Purified anti-Acetyl-CoA Carboxylase 1 Antibody, 25μg

Acetyl-CoA carboxylase (ACC) is a cytosolic enzyme that catalyzes carboxylation of acetyl-CoA to malonyl-CoA, the initiating and rate-limiting step in fatty acid biosynthesis. Two isoforms of ACC exist that show tissue-specific distribution. ACC1 is predominantly expressed in lipogenic tissues, while ACC2 is found in oxidative tissues. In response to depletion of cellular ATP, AMPK phosphorylates ACC1 at Ser79, resulting in loss of enzymatic activity. ACC1 is a clinical target for various metabolic diseases, and also contributes to metabolic reprogramming in tumor cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human acetyl-CoA carboxylase 1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: -Clone W19216A has a weak affinity for mouse acetyl-CoA carboxylase 1; we therefore do not recommend this clone for detecting mouse acetyl-CoA carboxylase -This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either Triton X-100 or methanol. Both methods were compatible with staining of acetyl-CoA carboxylase 1. -This clone may cross react with acetyl-CoA carboxylase 2.
RRID: AB_2888906 (BioLegend Cat. No. 942201) AB_2888906 (BioLegend Cat. No. 942202)
Structure: Acetyl-CoA carboxylase 1 is a 2,346 amino acid protein with a predicted molecular weight of 266 kD.
Distribution: Lipogenic tissue/Cytosol
Function: Fatty acid biosynthesis
Biology Area: Cell Biology, Mitochondrial Function
Molecular Family: Enzymes and Regulators
Antigen References: 1. Chen L, et al. 2019. Expert Opin. Investig. Drugs. 28:917. 2. Munday MR, et al. 1999. Adv. Enzyme Regul. 39:205. 3. Saddik M, et al. 1993. J Biol Chem. 268:25836.
Gene ID: 31
UniProt: View information about Acetyl-CoA Carboxylase on UniProt.org
Clone: W19216A
Regulatory Status: RUO
Other Names: Acetyl-CoA Carboxylase Alpha, Acetyl-Coenzyme A Carboxylase Alpha, ACC1, ACCA
Isotype: Rat IgG2a, κ