Biolegend, 942302, Purified anti-CHD4 Antibody, 100μg

Chromatin-helicase-DNA-binding (CHD) proteins are highly conserved chromatin-remodeling enzymes that can function in transcriptional activation, repression, and DNA repair. CHD family members are generally components of multi-subunit complexes, with interacting partners dictating repressive or activating function. CHD4 is a critical component of the nucleosome remodeling and histone deacetylation (NuRD) complex. Aberrant expression of CHD4 is linked to multiple types of cancer and functions as an oncogene in breast and colorectal cancers. Mutations in CHD4 cause Sifrim-Hitz-Weiss syndrome.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human CHD4 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: During product development testing for western blot, we observed additional bands below the predominant, full-length CHD4 band. These additional bands were also detected using a CHD4 antibody raised using a separate immunogen. We therefore believe these bands represent CHD4 degradation products or additional CHD4 isoforms. This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either Triton X-100 or methanol. While both methods were compatible with robust CHD4 staining, we observed punctate, extranuclear staining of cells permeabilzed with Triton X-100. We therefore do not recommend this method when using the clone for ICC.
RRID: AB_2888898 (BioLegend Cat. No. 942301) AB_2888898 (BioLegend Cat. No. 942302)
Structure: CHD4 is a 1,912 amino acid protein with a predicted molecular weight of 218 kD.
Distribution: Ubiquitously expressed/Nucleus
Function: Chromatin modifying enzyme
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: Arends T, et al. 2019. Proc Natl Acad Sci U S A. 116:10927-10936. Xia L, et al. 2017. Cancer Cell. 31:653-668. Weiss K, et al. 2016. Am J Hum Genet. 99:934-941.
Gene ID: 1108
UniProt: View information about CHD4 on UniProt.org
Clone: W18333A
Regulatory Status: RUO
Other Names: Chromodomain Helicase DNA Binding Protein 4, Mi2-β
Isotype: Rat IgG2a, κ