Biolegend, 942402, Purified anti-Filamin B Antibody, 100μg

Filamin B is an actin-binding protein that functions as a scaffold protein linking the cytoskeleton to the plasma membrane. It is required for receptor-mediated signal transduction and is a key regulator of intracellular signaling through its cross-linking and anchoring of actin, which allows for communication between the cell membrane, transmembrane receptors, and the cytoskeletal network. As a scaffold protein,  it plays a vital role in membrane stability, cell migration, ion channel transport, mechano-transduction, and tissue morphogenesis. In endothelial cells, Filamin B is crucial to vascular development and endothelial cell motility and migration, which it accomplishes through its role as a scaffold between RAC-1 and VAV-2. Vital to skeletal development, cartilage development, and mineral absorption, FLNB (gene encoding Filamin B) mutations or deficiencies result in skeletal malformations, including joint dislocation, scoliosis, spondylocarpotarsal synostosis, Larsen syndrome, and atelosteogenesis. In cancers, Filamin B may regulate tumorigenesis and cancer cell apoptosis, transcription, and alternative splicing. Deficiencies in FLNB expression result in tumor growth and metastasis.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Synthetic human Filamin B peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. No staining was observed with either method. This clone failed to adequately enrich Filamin B from HeLa whole cell extracts.
RRID: AB_2890863 (BioLegend Cat. No. 942401) AB_2890863 (BioLegend Cat. No. 942402)
Structure: Filamin B is a 2,602 amino acid protein with an expected molecular weight of 278 kD.
Distribution: Ubiquitously expressed/ Cytoskeleton
Function: Actin binding
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure
Molecular Family: Cytoskeletal Proteins
Antigen References: 1. Baudier J, et al. 2018. J. Cell. Sci. 131:8. 2.Del Valle-Perez B, et al. 2009. J. Biol. Chem. 285:10748. 3. Lu J, et al. 2007. Hum. Mol. Genet. 16:1661. 4. Ma H, et al. 2020. Oncol. Rep. 43:1536
Gene ID: 2317
UniProt: View information about Filamin B on UniProt.org
Clone: W19323A
Regulatory Status: RUO
Other Names: FLNB, ABP-280, ABP-278, TABP, TAP, Larsen Syndrome 1 (Autosomal Dominant), Actin Binding Protein 278, Thyroid Autoantigen, Filamin Homolog 1, Filamin B, Beta ABP-280 Homolog, Beta-Filamin, Filamin-B, Filamin-3
Isotype: Rat IgG2a, λ