Biolegend, 943201, Purified anti-SARS-CoV-2 S Protein S2 Antibody, 25μg

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits, S1 and S2. S1 is responsible for binding to the host cell via ACE2 receptor and the S2 subunit is responsible for fusion with the host cell.
25μg
Verified Reactivity: SARS-CoV-2
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant SARS-CoV-2 S protein corresponding to S2 subunit
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For the Direct ELISA application, a concentration of 3.7 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2888742 (BioLegend Cat. No. 943201) AB_2888742 (BioLegend Cat. No. 943202)
Structure: Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 amino acids with a theoretical molecular weight of 141 kD, and consists of S1 and S2 subunits. The S2 domain is 587 amino acids long with a predicted molecular weight of 64 kD.
Distribution: Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
Function: Mediates fusion between virus and host cell membranes
Interaction: ACE2
Ligand/Receptor: ACE2
Biology Area: COVID-19
Antigen References: 1. Walls AC, et al. 2020. Cell. 181(2):281-292. 2. Yan R, et al. 2020. Science. 367 (6485):1444-1448. 3. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263. 4. Shang J, et al. 2020. PNAS. 117(21):11727-11734
Gene ID: 43740568
UniProt: View information about Viral Protein on UniProt.org
Clone: A20085C
Regulatory Status: RUO
Other Names: Spike glycoprotein, S glycoprotein (E2)
Isotype: Mouse IgG2b, κ