Biolegend, 944402, Purified anti-SARS-CoV-2 S Protein S1 Antibody, 100μg

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 for fusion with the host cell.
100μg
Verified Reactivity: SARS-CoV-2
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant protein corresponding to the S1 subunit of SARS-CoV-2 S protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ELISA - Verfied
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For ELISA applications, a concentration of 65.4 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: BioLegend offers multiple clones that recognize SARS-CoV-2 S protein S1. Clone A20103H displayed the strongest performance for western blot of all clones validated for the application.
RRID: AB_2890872 (BioLegend Cat. No. 944401) AB_2890872 (BioLegend Cat. No. 944402)
Structure: Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
Distribution: Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
Function: Host cell surface receptor binding
Interaction: ACE2
Ligand/Receptor: ACE2
Biology Area: COVID-19
Antigen References: Walls AC, et al. 2020. Cell. 181:281-292. Yan R, et al. 2020. Science. 367:1444-1448. Wrapp D, et al. 2020. Science. 367:1260-1263. Shang J, et al. 2020. Proc Natl Acad Sci U S A. 117:11727-11734.
Gene ID: 43740568
UniProt: View information about Viral Protein on UniProt.org
Clone: A20103H
Regulatory Status: RUO
Other Names: Spike glycoprotein, Sglycoprotein (E2)
Isotype: Rat IgG2b, κ