Biolegend, 944903, Ultra-LEAF™ Purified anti-human CD137 (4-1BB) Antibody, 100μg

TNFRSF9, also known as 4-1BB and CD137, is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It is a costimulatory receptor similar to OX40, CD27, CD30, and CD40. The interaction of TNFRSF9 and its ligand, TNFSF9, leads to the activation of cytotoxic T cells, reactivation of anergic T lymphocytes, and induces T cell proliferation. TNFRSF9/TNFSF9 preferentially enhances the expansion of CD8+ T cells rather than CD4+ T cells, through the increase of IL-2Rα (CD25) and IL-2 expressions in CD8+ T cells. Among the TNFRSF members, TNFRSF9 is the most potent inducer of IL-2Rα expression on CD8+ T cells. In vivo studies with TNFSF9 knockout mice and with the agonistic anti-TNFRSF9 monoclonal antibody showed that TNFRSF9/TNFSF9 interaction keeps the optimal CD8+ T cell-mediated immune response against viral infections and tumors. The mouse TNFRSF9 has 60% identity to the human receptor at the amino acid level.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD137
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding between human TNFSF9 (Cat. No. 750006) and human TNFRSF9/4-1BB (Cat. No. 772606). The ND50 range: 0.01 - 0.06 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Manes TD, et al. 2022. Front Immunol. 13:1016361. PubMed
RRID: AB_2904454 (BioLegend Cat. No. 944903) AB_2904454 (BioLegend Cat. No. 944904)
Structure: Dimer
Distribution: Activated T cells, natural killer cells, macrophages, neutrophils, dendritic cells, eosinophils, Tregs, activated B cells, mast cells, and endothelial cells in tumor capillaries
Function: Proliferation of T cells, activates their effector functions, survival, and establishes immunological memory
Interaction: Activated antigen presenting cells, macrophages, B cells, B cell lymphoma, dendritic cells, a small fraction of T cells, epithelial, and endothelial cells.
Ligand/Receptor: TNFSF9 and CD137L
Cell Type: B cells, Dendritic cells, Endothelial cells, Mast cells, T cells, Tregs
Biology Area: Immuno-Oncology, Immunology, Protein Trafficking and Clearance
Molecular Family: CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References: Pollok KE, et al. 1994. Eur. J. Immunol. 24:367. Watts TH. 2005. Annu. Rev. Immunol. 23:23-68. Zhu Y, et al. 2007. Blood 109:4882. Locatelli F. 2009. Blood 11:2208. Wang J, et al. 2010. J. Immunol. 185:7654. Eun SY, et al. 2015. J. Immunol. 1:134-41. Spencer AJ, et al. 2014. PLoS One. 9:e105520. Yi L, et al. 2014. PLoS One 1:e86337.
Gene ID: 3604
UniProt: View information about CD137 on UniProt.org
Clone: W18063F
Regulatory Status: RUO
Other Names: CD137, CDw137, 4-1BB, 4-1BB ligand receptor, T cell antigen ILA, T cell antigen 4-1BB homolog
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.