Biolegend, 945202, Purified anti-Merlin (NF2) Antibody, 100μg

Moesin-ezrin-radixin-like protein (Merlin) is a tumor suppressor protein encoded by the Neurofibromin 2 (NF2) gene. It functions as a scaffold linking the plasma membrane and the cytoskeleton, and plays a crucial role in regulating cell polarization and morphology. Merlin functions as a major upstream activator of the Hippo signaling pathway, where it promotes Lats1/2 degradation, leading to increased nuclear localization of the YAP/TAZ transcription factors. Mutations and deletions within the NF2 gene cause neurofibromatosis type 2, a tumorigenic disease of the brain.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Synthetic peptide corresponding to human Merlin.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100, and methanol-fixed and permeabilized HeLa cells. None of the methods were compatible with Merlin staining. This clone is not predicted to cross-react with other Merlin homologs due to poor sequence conservation between the immunizing peptide and the corresponding region in each homolog.
RRID: AB_2892510 (BioLegend Cat. No. 945201) AB_2892510 (BioLegend Cat. No. 945202)
Structure: Merlin is a 595 amino acid protein with a predicted molecular weight of 70 kD.
Distribution: Ubiquitously expressed/Cytosol
Function: Tumor suppression, apoptosis
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References: Li W, et al. 2010. Cell. 140:477-490 Petrilli A, et al. 2016. Oncogene. 35:537-548. White S, et al. 2019. Dev Cell. 49:425-443.E9.
Gene ID: 4771
UniProt: View information about Merlin on UniProt.org
Clone: W19231A
Regulatory Status: RUO
Other Names: Neurofibromin 2, NF2, Merlin, Schwannomin, SCH, Neurofibromin 2 (Bilateral Acoustic Neuroma), Moesin-Ezrin-Radixin-Like Protein, Moesin-Ezrin-Radixin Like, Schwannomerlin, BANF, ACN
Isotype: Rat IgG2b, κ