Biolegend, 948301, Purified anti-CREB1 Antibody, 25μg

Cyclic AMP (cAMP)-response element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor binding to the cAMP response element (CRE) in response to certain stimuli. CREB1 contains a basic leucine zipper (bZIP) DNA-binding domain, 1 dimerization domain, and 9 serine residues in 1 kinase inducible domain phosphorylated by multiple kinases, including Ca2+/CaM-dependent kinase (CaMK) II and IV, protein kinase A (PKA), protein kinase C (PKC), mitogen/stress-activated kinase (MSK), ribosomal S6 kinase (RSK), AKT, and MAPKAP kinase 2 (MK2). Both the protein levels and activation of CREB1 are vital for maintaining cellular homeostasis in physiological and pathological conditions.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant human CREB1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone recognizes endogenous CREB1 proteins at 43 kD in both human and mouse cell lysates.
RRID: AB_2894537 (BioLegend Cat. No. 948301) AB_2894537 (BioLegend Cat. No. 948302)
Structure: CREB1 contains 327 amino acids at a predicted molecular weight at 35 kD.
Distribution: Ubiquitously expressed /Nucleus
Function: Under physiological conditions, CREB1 protein maintain cellular survival, proliferation, anti-inflammatory immune responses and modulates the development of the nervous system by serving as a neuroprotective role. Under pathological conditions, increased level of CREB1 associates with acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin’s lymphoma, chronic lymphatic leukemia (CLL), melanoma, hepatocellular, renal cell, ovarian, prostate, lung, gastric, esophageal, pancreatic, and breast tumors. CREB1 is also involved in the aging process by regulating the downstream signals of mitochondria dysfunction.
Interaction: CREB1 interacts with CREBBP, CRTC2, EP300, CRTC1, JUN, AKT1, CAMK4, FOS, RPS6KA1, RPS6KA3, CaMKII, CaMKIV, PKA, PKC, MSK, RSK, AKT, and MK2.
Cell Type: B cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, NK cells, T cells
Biology Area: Cell Biology, Transcription Factors
Antigen References: Andre S, et al. 2020. Cell Mol Life Sci. 77:4049-4067 Pei-Hsuan L, et al. 2020. Diagnostics. 10:295 Kensuke S, et al. 2011. J Neurochem. 116:1-9 Andy YW, et al. 2010. J Immunol. 185:6413-6419 TA, et al. 2002. EMBO J. 21:53-63
Gene ID: 1385
UniProt: View information about CREB1 on UniProt.org
Clone: A18233D
Regulatory Status: RUO
Other Names: CREB, CREB-1, cAMP responsive element binding protein 1
Isotype: Mouse IgG2a, κ