Biolegend, 949402, Purified anti-TACC3 Antibody, 100μg

TACC3, also referred to as transforming acidic coiled-coil containing protein 3 or ERIC1, is involved in the process of mitotic spindle assembly and stabilization. TACC3 forms a complex with ch-TOG/clathrin to stabilize the kinetochore fibers of the mitotic spindle. It acts as an inter-microtubule bridge and maintains kinetochore tension. TACC3 is frequently overexpressed in many cancers and may serve as a therapeutic and prognostic marker in breast cancer. Multiple solid tumors have been described where TACC3 is found as a fusion with various FGFR family members, which results in constitutive receptor tyrosine kinase activity of the FGFR partner.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human TACC3 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa cells fixed with methanol or 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. All three methods were compatible with TACC3 staining.
RRID: AB_2910516 (BioLegend Cat. No. 949401) AB_2910516 (BioLegend Cat. No. 949402)
Structure: TACC3 is an 838 amino acid protein with a predicted molecular weight of 90 kD.
Distribution: Ubiquitously expressed/cytoplasm and mitotic spindle assembly
Function: Cell cycle, cell division, mitosis, differentiation
Biology Area: Cell Biology
Molecular Family: Microtubules
Antigen References: Gangisetty, et al. 2004. Oncogene. 23:2559. Costa R, et al. 2016. Oncotarget. 7:55924. Ding, et al. 2017. Cytoskeleton. 74:369. Huo Q, et al. 2021. Front. Genet. 12:640078.
Gene ID: 10460
UniProt: View information about TACC3 on UniProt.org
Clone: W18281B
Regulatory Status: RUO
Other Names: Transforming acidic coiled-coil containing protein 3 , ERIC1
Isotype: Rat IgG2a, κ