Biolegend, 949801, Purified anti-Caspase-3 Antibody, 25μg

Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8, and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human Caspase-3 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody recognizes endogenous human Caspase-3 proteins in both total and spliced forms from Jurkat cell lysates treated with or without staurosporine.
RRID: AB_2904464 (BioLegend Cat. No. 949801) AB_2904464 (BioLegend Cat. No. 949802)
Structure: Caspase 3 is a 277 amino acid protein with a molecular mass of 32 kD.
Distribution: Nucleus and cytosol
Function: Caspase 3 regulates programmed cell death (apoptosis)
Interaction: Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo
Cell Type: B cells, Endothelial cells, Fibroblasts, Monocytes, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Neuroscience
Antigen References: Goldberg YP, et al. 1996. Nat Genet. 13:442-9. Fernandes-Alnemri T, et al. 1996. Proc Natl Acad Sci U S A. 93:7464-9. Slee EA, et al. 2001. J Biol Chem. 276:7320-6. Woo M, et al. 2003. Nat Immunol. 4:1016-22.
Gene ID: 836
UniProt: View information about Caspase-3 on UniProt.org
Clone: W20054B
Regulatory Status: RUO
Other Names: Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
Isotype: Rat IgG2a, κ