CST, 20223T, SNAP25 (F1A9G) Mouse Monoclonal Antibody

Monoclonal Antibody for studying SNAP25. Validated for WB,WB,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SNAP25 (F1A9G) Mouse Monoclonal Antibody (CST #20223) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Frozen): 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 54626 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SNAP25 (F1A9G) Mouse Monoclonal Antibody recognizes endogenous levels of total SNAP25 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SNAP25 protein. Background The 25 kDa synaptosome-associated protein (SNAP25) is a target membrane soluble, N-ethylmaleimide-sensitive factor attachment protein receptor (t-SNARE) that is found on neuronal presynaptic membranes. SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca -dependent exocytosis (1). This complex is responsible for exocytosis of the neurotransmitter γ-aminobutyric acid (GABA). Neurotransmitter release is inhibited by proteolysis of SNAP25 by botulinum toxins A and E (2). SNAP25 plays a secondary role as a Q-SNARE involved in endosome fusion; the protein is associated with genetic susceptibility to attention-deficit hyperactivity disorder (ADHD) (3). Alternate Names bA416N4.2; CMS18; dJ1068F16.2; FLJ23079; resistance to inhibitors of cholinesterase 4 homolog; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SNAP25; SNP25; SUP; Super protein; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; synaptosomal-associated protein, 25kDa; synaptosome associated protein 25; synaptosome associated protein 25kDa Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Mouse IgG2b kappa