CST, 20289S, GCSAM/HGAL Antibody

Polyclonal Antibody for studying GCSAM. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, GCSAM/HGAL Antibody (CST #20289) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GCSAM/HGAL Antibody recognizes endogenous levels of total GCSAM/HGAL protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GCSAM/HGAL protein. Antibodies are purified by peptide affinity chromatography. Background Germinal center-associated signaling and motility protein/Human germinal center-associated lymphoma (GCSAM/HGAL) is reported to be a marker for germinal center B cells and germinal center-derived B-cell lymphomas (1). Research studies have shown that GCSAM is an adaptor protein anchored to the plasma membrane via myristoyl and palmitoyl linkages and is phosphorylated on multiple tyrosine residues by Syk and Lyn upon engagement of the B cell antigen receptor (BCR), which promotes its interaction with Grb2 and Syk. Binding of GCSAM to Syk positively regulates BCR signaling by enhancing Syk kinase activity (2-5). In addition to regulating antigen-induced BCR signaling in germinal center B cells, research studies have demonstrated that GCSAM restricts motility of B cells within the germinal center microenvironment through activation of RhoA signaling (6). Through regulation of these two processes, GCSAM has been implicated in the differentiation of germinal center B cells to either plasma cells or memory B cells (7). Alternate Names GAL; GCAT2; GCET2; GCSAM; germinal center associated signaling and motility; germinal center B cell associated-protein 2; Germinal center B-cell-expressed transcript 2 protein; germinal center expressed transcript 2; Germinal center-associated lymphoma protein; Germinal center-associated signaling and motility protein; hGAL; human germinal center-associated lymphoma; MGC40441 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit