CST, 2396S, MBP-Tag (8G1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MBP-Tag (8G1) Mouse Monoclonal Antibody (CST #2396) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MBP-Tag (8G1) Mouse Monoclonal Antibody detects recombinant proteins fused to maltose binding protein (MBP). Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with an MBP fusion protein. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. One commonly used system is the maltose-binding protein (MBP) (1). In this system, the target gene is inserted in the appropriate vector and expressed. Following purification using an amylose column, the MBP-target protein complex can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein (2). An antibody to the MBP tag can also be used to isolate or detect expression of the protein. Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1