CST, 2622S, GST-Tag Antibody

Polyclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, GST-Tag Antibody (CST #2622) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GST-Tag Antibody detects transfected GST fusion proteins. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a GST fusion protein. Antibodies are purified by protein A and GST affinity chromatography. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3). Specification REACTIVITY: All SENSITIVITY: Transfected Only SOURCE: Rabbit