CST, 32325S, IL2-R alpha/CD25 (PC61.5) Rat Monoclonal Antibody (PerCP-Cy5.5® Conjugate)

Monoclonal Antibody for studying IL2RA mouse. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, IL2-R alpha/CD25 (PC61.5) Rat Monoclonal Antibody (PerCP-Cy5.5^® Conjugate) (CST #32325) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.25 μg of antibody per test. Flow Cytometry (Live): 1:80 Storage Supplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity IL2-R alpha/CD25 (PC61.5) Rat Monoclonal Antibody (PerCP-Cy5.5 Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4 T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). Alternate Names CD25; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2-RA; Il2r; Il2ra; interleukin 2 receptor, alpha chain; Interleukin-2 receptor subunit alpha; Ly-4; Ly-43; MGC130561; OTTMUSP00000012002; p55; p55 chain Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG1 lambda