CST, 32380S, IRF-7 (E2U6L) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying IRF-7 mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IRF-7 (E2U6L) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #32380) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IRF-7 (E2U6L) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with mouse IRF-7 recombinant protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response to viral infection (6-8). IRF-7 is regulated at multiple serine phosphorylation sites near the carboxyl terminus, similar to IRF-3, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11). Alternate Names Interferon regulatory factor 7; IRF-7; Irf7; OTTMUSP00000029373 Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG