CST, 32523L, Whitlow/218 Linker (E3U7Q) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, Whitlow/218 Linker (E3U7Q) Rabbit Monoclonal Antibody (Biotinylated) (CST #32523) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:50 Storage Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity Whitlow/218 Linker (E3U7Q) Rabbit Monoclonal Antibody (Biotinylated) recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide. Background The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG