CST, 33908S, Ki-67 (D3B5) Rabbit Monoclonal Antibody (Alexa Fluor® 594 Conjugate)

Monoclonal Antibody for studying KI-67. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Ki-67 (D3B5) Rabbit Monoclonal Antibody (Alexa Fluor^® 594 Conjugate) (CST #33908) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Ki-67 (D3B5) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to residues near the central region of human Ki-67 protein. Background Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4). Alternate Names antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; KI-67; KI67; KIA; marker of proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG