CST, 34105S, alpha-Smooth Muscle Actin (D4K9N) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, alpha-Smooth Muscle Actin (D4K9N) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #34105) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:50 - 1:100 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity alpha-Smooth Muscle Actin (D4K9N) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle actin protein. Background Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as "non-muscle" actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1). Alternate Names AAT6; ACTA; ACTA2; actin alpha 2, smooth muscle; actin, alpha 2, smooth muscle, aorta; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha 2 actin; alpha smooth muscle Actin; Alpha-actin-2; alpha-cardiac actin; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; smooth muscle actin Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG