CST, 35467S, CD8 alpha (2.43) Rat Monoclonal Antibody (FITC Conjugate)

Monoclonal Antibody for studying CD8A mouse. Validated for Immunofluorescence (Frozen),Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD8 alpha (2.43) Rat Monoclonal Antibody (FITC Conjugate) (CST #35467) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.5 μg of antibody per test. Immunofluorescence (Frozen): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 Flow Cytometry (Live): 1:100 Storage Supplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD8 alpha (2.43) Rat Monoclonal Antibody (FITC Conjugate) recognizes endogenous levels of total CD8α protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1). Alternate Names BB154331; CD8 antigen, alpha chain; Cd8a; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2b