CST, 35665S, CD47 (E2V9V) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying CD47 mouse. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD47 (E2V9V) Rabbit Monoclonal Antibody (Alexa Fluor^® 555 Conjugate) (CST #35665) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity CD47 (E2V9V) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse CD47 recombinant protein. Background CD47 is a five-pass transmembrane protein expressed on all normal cells. It binds to the SIRPα that is expressed on myeloid cells, including macrophages, and neuronal cells in the central nervous system. Binding of CD47 to SIRPα promotes phosphorylation of tyrosine residues in the immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the SIRPα cytoplasmic tail, inhibiting macrophage phagocytosis toward CD47-expressing cells. In this way, CD47 serves as a "don't eat me" signal or a marker of "self", functioning as an innate immune checkpoint. Additionally, CD47 was reported to modulate lymphocyte cell activation and proliferation (1-3). CD47 is overexpressed in many types of cancer. The expression level of CD47 on cancer cells is negatively associated with the response to therapies, and low expression on tumor cells is associated with a better prognosis and survival. Reagents that can block CD47-SIRPα interaction are being actively pursued for therapeutic applications (4,5). In addition to SIRPα, other proteins have been reported to bind to CD47. Thrombospondin-1 (TSP1) competes with SIRPα to bind to CD47 in the extracellular region and activates signaling pathways downstream of CD47 (6). CD47 can laterally associate with VEGFR2, FAS, and certain integrins in different contexts, and influences their downstream signaling (7-9). CD47 can be shed from the cell surface by proteolytic cleavage. In addition, CD47 is present on extracellular vesicles including exosomes, suggesting additional extracellular signaling potential (10). Alternate Names 9130415E20Rik; AA407862; AI848868; AW108519; B430305P08Rik; Cd47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); IAP; Integrin-associated protein; Itgp; Leukocyte surface antigen CD47; OTTMUSP00000015907; Rh-related antigen Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG