CST, 40399SF, MYH11 (E4Q4L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MYH11. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, MYH11 (E4Q4L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #40399) is ready to ship. Product Usage Information This product is the carrier free version of product #79479. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 79479 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MYH11 (E4Q4L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MYH11 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu639 of human MYH11 protein. Background encodes the smooth muscle myosin heavy chain (SMMHC), a major specific contractile protein produced in smooth muscle (1). Mutations in cause medial degeneration of the aorta with very low smooth muscle cell (SMC) content. Disease symptoms include aortic stiffness, aneurysm/dissection, and ductus arteriosus (2). One of the most frequent chromosomal translocations associated with acute myeloid leukemia (AML) is the fusion of , encoding the core binding protein CBFβ transcriptional factor, to (3). This gene fusion event results in a chimeric protein that suppresses the transactivation mediated by CBF/AML-1, impairs the process of normal hematopoietic differentiation (4,5), and creates preleukemic myeloid progenitors that can be targets for AML transformation (6). Alternate Names AAT4; DKFZp686D10126; DKFZp686D19237; epididymis secretory sperm binding protein; FAA4; FLJ35232; KIAA0866; MGC126726; MGC32963; MYH11; Myosin heavy chain 11; Myosin heavy chain, smooth muscle isoform; Myosin-11; myosin, heavy chain 11, smooth muscle; myosin, heavy polypeptide 11, smooth muscle; SMHC; SMMHC Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 270 Source/Isotype: Rabbit IgG