CST, 4043S, BAP31 Antibody

Polyclonal Antibody for studying BAP31. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, BAP31 Antibody (CST #4043) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:25 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BAP31 Antibody detects endogenous levels of total BAP31 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Arg71 of BAP31. Antibodies were purified by peptide affinity chromatography. Background BAP31 ( -cell receptor- ssociation rotein 31) is a transmembrane protein associated with the endplasmic reticulum (ER) and ER-Golgi intermediates and has been implicated in protein trafficking and apoptosis (1,2). During apoptosis Bap31 is cleaved by caspase-8 at two carboxy-terminal sites which can then direct apoptotic signals between the ER and mitochondria (2-4). Association of BAP31 with the anti-apoptotic proteins Bcl-2 or Bcl-xL could function to regulate this ER-mitochondrial pathway (2,5). Several studies have shown that BAP31 can control the trafficking of select proteins between the ER and Golgi apparatus and can affect the transport of proteins to the cell surface (6-10). Alternate Names 6C6 antigen; 6C6-AG; 6C6-AG tumor-associated antigen; B cell receptor associated protein 31; B-cell receptor-associated protein 31; BAP31; BCAP31; BCAP31 iso2; BCR-associated protein 31; BCR-associated protein Bap31; CDM; DDCH; DXS1357E; p28; p28 Bap31; Protein CDM Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit