CST, 40471S, Transgelin/TAGLN Antibody

Polyclonal Antibody for studying TAGLN. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Transgelin/TAGLN Antibody (CST #40471) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Transgelin/TAGLN Antibody recognizes endogenous levels of total Transgelin/TAGLN protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly171 of human Transgelin/TAGLN protein. Antibodies are purified by peptide affinity chromatography. Background Transgelin (TAGLN, SM22α) is an actin-binding protein of the calponin family. It contains an amino-terminal calponin homology domain and a carboxy-terminal calponin-like module domain required for actin binding (1,2). is a TGF-β inducible gene that is abundantly expressed in visceral and vascular smooth muscle cells, and is often used as an early marker of smooth muscle differentiation (3-5). TAGLN expression has also been shown in brain pericytes, providing use in studying the blood brain barrier (6). Altered levels of TAGLN are seen in multiple cancer types, including colorectal, breast, and prostate cancer (4). Alternate Names 22 kDa actin-binding protein; DKFZp686B01212; DKFZp686P11128; epididymis secretory sperm binding protein; Protein WS3-10; SM22; SM22-alpha; SMCC; Smooth muscle protein 22-alpha; TAGL; TAGLN; TAGLN1; Transgelin; transgelin variant 2; WS3-10 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 23 SOURCE: Rabbit