CST, 40649T, Pyrin/MEFV (E2E8U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying pyrin. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Pyrin/MEFV (E2E8U) Rabbit Monoclonal Antibody (CST #40649) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Pyrin/MEFV (E2E8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total Pyrin/MEFV protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Pyrin/MEFV protein. Antigen mapping indicates that this antibody and Pyrin/MEFV (E9U8M) Rabbit mAb #58525 have different epitopes. Background Pyrin is encoded by the gene that is frequently mutated in patients with familial Mediterranean fever (FMF), an autoinflammatory disorder that causes recurrent fevers, usually also accompanied by pain in the abdomen, chest, and joints. It is expressed mainly in cells of the innate immune system, such as granulocytes, eosinophils, monocytes, and dendritic cells (1,2). Pyrin can form inflammasomes with ASC and Casp1, and this is regulated by RhoA guanosine triphosphatase (GTPase). RhoA activation leads to PKN1/2-mediated phosphorylation of Ser208 and Ser242 on Pyrin (Ser205 and Ser241 on mouse Pyrin, respectively). The chaperone 14-3-3 proteins bind to the phosphorylated Pyrin and keep it in an inactive state. Agents that inactivate RhoA, such as certain bacterial toxins, cause a decrease in PKN1/2 activity and Pyrin phosphorylation. This, in turn, releases Pyrin from the inhibitory 14-3-3 proteins and allows Pyrin to form an active inflammasome (3-6). Alternate Names FMF; Marenostrin; Mediterranean fever; Mediterranean fever protein; MEF; MEFV; MEFV innate immuity regulator, pyrin; MEFV, pyrin innate immunity regulator; MGC126560; MGC126586; PAAND; Pyrin; TRIM20 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG