CST, 40656SF, CD177 (F9O5U) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD177. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD177 (F9O5U) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #40656) is ready to ship. Product Usage Information This product is the carrier free version of product #18991. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 18991 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD177 (F9O5U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD177 protein. Non-specific staining was observed in human normal and neoplastic kidney by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD177 protein. Background Cluster of differentiation 177 antigen (CD177, also known as human neutrophil alloantigen 2a and NB1), is a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated transmembrane protein expressed primarily on the surface of neutrophils (1,2). Expression of CD177 can be induced by granulocyte-colony-stimulating factor and during pregnancy or bacterial infection (3-5). CD177 mediates adhesion and transendothelial migration of neutrophils via interaction with CD31/PECAM-1 and integrin β2 on endothelial cells (6,7). CD177 also promotes the cell surface expression of proteinase 3 (PR3), which may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (8-10). In association with CD11b/ITGAM, integrin β2, and PR3, CD177 promotes activation of TNF-α primed neutrophils including degranulation and superoxide production (11).  is a polymorphic gene that has been linked to several important clinical diseases, including polycythemia vera, Wegener's granulomatosis, and immune mediated neonatal neutropenia (12-14). Alternate Names CD177; CD177 antigen; CD177 molecule; cell surface receptor; HNA-2a; HNA2A; Human neutrophil alloantigen 2a; NB1; NB1 glycoprotein; NB1 GP; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV-1; PRV1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 16, 30, 48, 65 Source/Isotype: Rabbit IgG