CST, 40659S, SQLE Antibody

Polyclonal Antibody for studying SQLE. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SQLE Antibody (CST #40659) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SQLE Antibody recognizes endogenous levels of total SQLE protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro85 of human SQLE protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Squalene monooxygenase (SQLE), also known as squalene epoxidase, catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene (1,2) and is considered to be a critical rate-limiting enzyme in cholesterol biosynthesis downstream of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) (3). The N-terminal region of SQLE is anchored to the endoplasmic reticulum membrane via a re-entrant loop, and undergoes a conformational change in response to elevated cholesterol levels. This cholesterol-mediated conformational change promotes the interaction of SQLE with the E3 ligase MARCH6, leading to targeted ubiquitination and proteasomal degradation (4-6). Altered expression of SQLE is associated with perturbed cholesterol homeostasis and tumor progression, prompting investigation of the therapeutic potential of SQLE (7). Moreover, research studies have shown that cholesterol auxotrophy and subsequent squalene accumulation observed in certain cancers may prevent oxidative cell death and represent a targetable vulnerability to SQLE inhibitors (8,9). Alternate Names ERG1; FLJ30795; SE; SQLE; Squalene epoxidase; Squalene monooxygenase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit