CST, 40672S, CXXC1 (D1R5R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CXXC1. Validated for WB,IP,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, CXXC1 (D1R5R) Rabbit Monoclonal Antibody (CST #40672) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 CUT&RUN: 1:100 CUT&Tag: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, CUT&RUN, CUT&Tag Specificity / Sensitivity CXXC1 (D1R5R) Rabbit Monoclonal Antibody recognizes endogenous levels of total CXXC1 protein. In Western blot analysis, this antibody detects a 32kDa protein of unknown identity. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly107 of human CXXC1 protein. Background The CXXC finger protein 1 (CXXC1, CGBP, CFP1) is a key subunit of the human SET1 histone methyltransferase complex (1,2) that methylates histone H3 at Lys4 to create a mark of transcriptionally active promoters (3,4). CXXC1 is enriched at CpG islands where it selectively binds non-methylated CpG motifs to provide a link between global H3K4 methylation and CpG islands (5). Research studies have revealed a role for CXXC1 in the maintenance of cytosine methylation through direct interaction with DNMT1 (6-9). The epigenetic functions of CXXC1 are critical for normal embryonic development. Targeted deletion of the murine gene results in early embryonic lethality while Cxxc1- embryonic stem (ES) cells exhibit increased apoptosis and fail to undergo differentiation following withdrawal of leukemia inhibitory factor LIF (6). Alternate Names 2410002I16Rik; 5830420C16Rik; CFP1; CGBP; CpG binding protein; CpG-binding protein; CXXC finger 1 (PHD domain); CXXC finger protein 1; CXXC-type zinc finger protein 1; CXXC1; DNA-binding protein with PHD finger and CXXC domain; hCGBP; HsT2645; PCCX1; PHD finger and CXXC domain-containing protein 1; PHF18; SPP1; ZCGPC1; zinc finger, CpG binding-type containing 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG