CST, 40758T, Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CASC5 (Thr943) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit Monoclonal Antibody (CST #40758) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 72586 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit Monoclonal Antibody recognizes endogenous levels of CASC5 protein only when phosphorylated at Thr943 or Thr1155. The protein sequences surrounding these two sites are identical. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr943 of human CASC5 protein. Background Kinetochores are mitotic structures that form on centromeres and attach to mitotic spindle microtubules. Kinetochore attachment to microtubules regulates chromosome segregation and progression through mitosis. Unattached kinetochores signal to the spindle assembly checkpoint (SAC) machinery, arresting cells in mitosis (1). CASC5, also known as Knl1 or Blinkin, is the largest subunit of the Knl1-Mis12-Ndc80 complex (KMN) network, a structural component of kinetochores required for microtubule binding. CASC5 functions in the formation of kinetochore-microtubule attachments, chromosome segregation, and in activating the SAC. CASC5 has been implicated in human diseases, including leukemia and microcephaly (2). Activation of the SAC is regulated in part by mitotic phosphorylation of CASC5 at several sites, including Ser24, Ser60, Thr943, and Thr1155 (3,4). The sequences surrounding Thr943 and Thr1155 are identical. Alternate Names AF15q14; ALL1-fused gene from chromosome 15q14; ALL1-fused gene from chromosome 15q14 protein; Blinkin; blinkin, bub-linking kinetochore protein; Bub-linking kinetochore protein; cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CASC5; CT29; D40; hKNL-1; hSpc105; KIAA1570; kinetochore null 1 homolog; Kinetochore scaffold 1; Kinetochore-null protein 1; KNL1; MCPH4; microcephaly, primary autosomal recessive 4; PPP1R55; Protein CASC5; Protein D40/AF15q14; protein phosphatase 1, regulatory subunit 55; Spc7 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 265 Source/Isotype: Rabbit IgG