CST, 43590S, mCherry (E5D8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying mCherry bacteria. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, mCherry (E5D8F) Rabbit Monoclonal Antibody (CST #43590) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity mCherry (E5D8F) Rabbit Monoclonal Antibody detects mCherry-tagged proteins (either N-terminal tagged or C-terminal tagged) exogenously expressed in cells. Please note that the mCherry tags add approximately 28kDa to the molecular weight of the fusion protein. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a full length recombinant mCherry protein. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. mCherry is a monomeric, fluorescent, photostable red dye that was isolated from and further engineered from the sea anemone (1). Its peak fluorescent excitation is at 587Â nm and emission 610Â nm (1). Alternate Names mCherry; MCherry fluorescent protein Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG