CST, 4392S, PIAS4 (D2F12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PIAS4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PIAS4 (D2F12) Rabbit Monoclonal Antibody (CST #4392) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PIAS4 (D2F12) Rabbit Monoclonal Antibody detects endogenous levels of total PIAS4 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys59 of human PIAS4 protein. Background The protein inhibitor of activated Stat (PIAS) proteins, which include PIAS1, PIAS3, PIASx, and PIASy, were originally characterized based on their interaction with the Stat family of transcription factors (1,2). PIAS1, PIAS3, and PIASx interact with and repress Stat1, Stat3, and Stat4, respectively (1-3). Deletion of PIAS1 leads to inhibition of interferon-inducible genes and increased protection against infection (4). The PIAS family contains a conserved RING domain that has been linked to a function as a small ubiquitin-related modifier (SUMO) ligase, coupling the SUMO conjugating enzyme Ubc9 with its substrate proteins (5,6). Numerous studies have now shown that PIAS family members can regulate the activity of transcription factors through distinct mechanisms, including NF-κB (7,8), c-Jun, p53 (5,9), Oct-4 (10), and Smads (11,12). The activity of PIAS1 is regulated by both phosphorylation and arginine methylation. Inflammatory stimuli can induce IKK-mediated phosphorylation of PIAS1 at Ser90, which is required for its activity (13). In addition, PRMT1 induces arginine methylation of PIAS1 at Arg303 following interferon treatment and is associated with its repressive activity on Stat1 (14). PIAS4, also known as PIASy, is a specific SUMO-E3 ligase for Ets-1 and represses Ets-1 dependent transcription (15). PIAS4 also alters the nuclear localization, reduces the transcriptional activity of C/EBPδ, and enhances cell proliferation and migration (16). Alternate Names E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PIAS-gamma; PIAS4; PIASG; PIASy; protein inhibitor of activated STAT 4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT, 4; RING-type E3 ubiquitin transferase PIAS4; zinc finger, MIZ-type containing 6; ZMIZ6 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG