CST, 4393T, FTH1 (D1D4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Ferritin H. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, FTH1 (D1D4) Rabbit Monoclonal Antibody (CST #4393) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity FTH1 (D1D4) Rabbit Monoclonal Antibody recognizes endogenous levels of total FTH1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FTH1 protein. Background Ferritin is a ubiquitous and highly conserved protein that plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form (1). The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron (2,3). It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain (FTH) and ferritin light chain (FTL), each having unique functions. FTHs catalyze the first step in iron storage, the oxidation of Fe(II), whereas FTLs promote the nucleation of ferrihydrite, enabling storage of Fe(III) (4). In addition to iron buffering, FTH also enhances thymidine biosynthesis (5). Serum ferritin levels indicate the amount of iron stored in the body and is the most sensitive test for anemia. It is markedly elevated in inflammation, malignancy, and iron overload disorders (6). Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases (7). Alternate Names apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; Ferritin heavy chain; ferritin heavy chain 1; Ferritin heavy chain, N-terminally processed; Ferritin-H; ferritin, heavy polypeptide 1; FHC; FRIH; FTH; FTH1; FTHL6; HFE5; MGC104426; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG