CST, 44006S, MSL2 (D4V2N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MSL2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MSL2 (D4V2N) Rabbit Monoclonal Antibody (CST #44006) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MSL2 (D4V2N) Rabbit Monoclonal Antibody recognizes endogenous levels of total MSL2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro210 of human MSL2 protein. Background Male-specific lethal 2-homolog (MSL2) is a component of the male-specific lethal (MSL) histone acetyltransferase complex, which contains MSL1, MSL2, MSL3, and MYST1. MYST1, also known as mammalian male absent on the first (MOF) and lysine acetyltransferase 8 (KAT8), is a member of the MYST (MOZ, YBF2, SAS2, and Tip60) family of histone acetyltransferases (1,2) and functions as the catalytic subunit of the MSL complex. The MSL complex is responsible for the majority of acetylation on histone H4 lysine 16 (3-5). In addition, as part of the MSL complex, MSL1 and MSL2 function as an E3 ubiquitin ligase to mono-ubiquitylate histone H2B on lysine 34 (6). The MSL complex plays a critical role in the regulation of transcription, DNA repair, autophagy, apoptosis, and embryonic stem cell pluripotency and differentiation (1,2,6,7). Loss of MSL activity leads to a global reduction in histone H4 lysine 16 acetylation, a common hallmark found in many human cancers. In particular, the reduction of MYST1 protein levels and histone H4 lysine 16 acetylation is associated with poor prognosis in breast, renal, colorectal, gastric, and ovarian cancers (1). Alternate Names E3 ubiquitin-protein ligase MSL2; E3 ubiquitin-protein transferase MSL2; FLJ10546; FLJ54913; KIAA1585; Male-specific lethal 2 homolog; male-specific lethal 2 homolog (Drosophila); Male-specific lethal 2-like 1; Male-specific lethal-2 homolog; Male-specific lethal-2 homolog 1; MSL complex subunit 2; MSL-2; MSL2; MSL2-like 1; MSL2L1; RING finger protein 184; RNF184 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG