CST, 4400S, MIB1 Antibody

Polyclonal Antibody for studying MIB1. Validated for Western Blotting. Highly specific and rigorously validated in-house, MIB1 Antibody (CST #4400) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MIB1 Antibody detects endogenous levels of total MIB1 protein. This antibody does not recognize the Ki-67 (MK167) protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MIB1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background Mindbomb homolog 1 (MIB1) is an E3 ligase that facilitates the ubiquitination and the subsequent endocytosis of the Notch ligands, Delta and Jagged (1,2). MIB1 appears to promote the ubiquitination and degradation of death-associated protein kinase (DAPK1) (3). Expression of MIB1 is seen in both adult and embryonic murine tissues (4). Recently, MIB1 was reported to regulate the extrinsic cell death pathway by binding to cellular FLICE-like inhibitory proteins (cFLIP-L and cFLIP-S), which reduces the interaction of caspase-8 with cFLIP and leads to cell death (5). MIB1 is also involved in T and marginal zone B (MZB) cell development in the lymphopoietic niches (6). Alternate Names DAPK-interacting protein 1; DIP-1; DIP1; DKFZp686I0769; DKFZp761M1710; E3 ubiquitin-protein ligase MIB1; FLJ90676; KIAA1323; LVNC7; MGC129659; MGC129660; MIB; MIB1; Mind bomb homolog 1; mindbomb E3 ubiquitin protein ligase 1; mindbomb homolog 1 (Drosophila); RING-type E3 ubiquitin transferase MIB1; ubiquitin ligase mind bomb; ubiquitin ligase protein MIB1; Zinc finger ZZ type with ankyrin repeat domain protein 2; ZZANK2; ZZZ6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 SOURCE: Rabbit