CST, 44056S, NSUN2 Antibody

Polyclonal Antibody for studying NSUN2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, NSUN2 Antibody (CST #44056) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NSUN2 Antibody recognizes endogenous levels of total NSUN2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val690 of human NSUN2 protein. Antibodies are purified by peptide affinity chromatography. Background Chemical modifications of RNA regulate many cellular processes. One particular RNA modification, 5-methylcytosine (5-mC), regulates ribosome assembly, translation, and RNA stability (1). In eukaryotes, this modification is added to RNA by the DNA methyltransferase homologue 2 protein (DNMT2; also known as TRDMT1), and also by members of the NOL1/NOP2/SUN domain (NSUN) family of proteins. NSUN proteins are putative S-adenosylmethionine (SAM)-dependent methyltransferases that carry out their enzymatic activity by utilizing two cysteine residues in their active sites (2). There are currently seven known members of this family, consisting of NOP2 (NSUN1) and NSUN2-7. NSUN2 is an 86 kDa member of the NSUN family that is predominantly localized to the nucleus and methylates a variety of tRNAs and other RNA substrates (3). NSUN2 has been found to play a role in the methylation of pre-tRNA , tRNA , tRNA , tRNA , and tRNA (1,4,5). It has also been found to methylate mRNAs and several ncRNAs, such as vtRNAs (6-8). Elevated NSUN2 protein levels have been reported in multiple types of human cancers, including breast, prostate, kidney, bladder, and liver, among others (9). The methyltransferase activity of NSUN2 is reduced upon Aurora B kinase-mediated phosphorylation at Ser139 (10), and there is potential for NSUN2 to be used as a therapeutic target or diagnostic marker for Aurora B linked cancers (9). Alternate Names 5-methycytoisine methyltransferase; cDNA FLJ76184, highly similar to Homo sapiens NOL1/NOP2/Sun domain family, member 2 (NSUN2), mRNA; FLJ20303; hTrm4; MISU; mRNA cytosine C(5)-methyltransferase; MRT5; Myc-induced SUN domain-containing protein; Myc-induced SUN-domain-containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family member 2; NOL1/NOP2/Sun domain family, member 2; NOP2/Sun domain family, member 2; NOP2/Sun RNA methyltransferase 2; NOP2/Sun RNA methyltransferase family member 2; NSUN2; RNA cytosine C(5)-methyltransferase NSUN2; SAKI; substrate of AIM1/Aurora kinase B; TRM4; tRNA (cytosine-5-)-methyltransferase NSUN2; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA cytosine C(5)-methyltransferase; tRNA methyltransferase 4 homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit