CST, 44058T, LCN2 (D4M8L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Lipocalin2. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, LCN2 (D4M8L) Rabbit Monoclonal Antibody (CST #44058) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunohistochemistry (Paraffin): 1:3000 - 1:12000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity LCN2 (D4M8L) Rabbit Monoclonal Antibody recognizes endogenous levels of total LCN2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala73 of human LCN2 protein. Background Lipocalin 2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), belongs to the lipocalin family. Proteins in this family exhibit considerable sequence diversity, but share a highly conserved structure composed of an eight-stranded antiparallel beta-barrel that encloses an internal ligand-binding site (1,2). LCN2 is considered as an iron transporter. LCN2 can bind bacterial siderophores and inhibits bacterial growth by iron depletion (3). It can also bind endogenous compounds like catechol in complex with iron (4). LCN2 was originally identified in neutrophils, and its expression was induced in many other tissues like kidney and liver in response to various pathologic states, such as infection, ischemia, and acute kidney injury. LCN2 is considered a biomarker for conditions like ischemic stroke, acute kidney injury, inflammatory and metabolic diseases (5-7). Alternate Names 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; Neutrophil gelatinase-associated lipocalin; NGAL; Oncogene 24p3; p25; siderocalin; siderocalin LCN2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG