CST, 44059S, TBP (D5C9H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TBP. Validated for WB,IP,ChIP,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, TBP (D5C9H) Rabbit Monoclonal Antibody (CST #44059) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Chromatin IP-seq: 1:100 CUT&RUN: 1:100 CUT&Tag: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq, CUT&RUN, CUT&Tag Specificity / Sensitivity TBP (D5C9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total TBP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TBP protein. Background TATA-binding protein (TBP) is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID (1-3). TFIID, which contains TBP and 13 TBP-associated factors (TAFs), contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II) that bind to a gene promoter during the initiation of transcription (1-3). Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex. Alternate Names GTF2D; GTF2D1; HDL4; MGC117320; MGC126054; MGC126055; SCA17; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TBP; TF2D; TFIID; Transcription initiation factor TFIID TBP subunit Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 35-45 Source/Isotype: Rabbit IgG