CST, 4406S, Phospho-Jak2 (Tyr1007) (D15E2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Jak2 (Tyr1007) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Jak2 (Tyr1007) (D15E2) Rabbit Monoclonal Antibody (CST #4406) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Jak2 (Tyr1007) (D15E2) Rabbit Monoclonal Antibody detects endogenous levels of Jak2 when phosphorylated at Tyr1007. This antibody can also detect double phosphorylation at Tyr1007 and Tyr1008. Cross-reactivity may be observed with phosphorylated Jak3 and Tyk2. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1007 of human Jak2. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names JAK-2; JAK2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; Tyrosine-protein kinase JAK2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG