CST, 44083T, nSMase2 (F4E1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMPD3. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, nSMase2 (F4E1D) Rabbit Monoclonal Antibody (CST #44083) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity nSMase2 (F4E1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total nSMase2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser527 of human nSMase2 protein. Background Sphingomyelinases (SMases) catalyze sphingomyelin hydrolysis to produce ceramide and phosphocholine (1). Ceramide is an important bioactive lipid triggering signal transduction in cell proliferation, apoptosis, and differentiation (1,2). Many SMases have been described and categorized based on their optimum pH activity, cation dependence, tissue distribution, and subcellular localization (1). These include a lysosomal acid SMase, a Zn -dependent secreted acid SMase, a membrane-bound Mg -dependent neutral SMase, a Mg -independent neutral SMase, and an alkaline SMase. nSMase1, also known as SMPD2, is a Mg -dependent neutral SMase widely expressed and predominantly localized to the endoplasmic reticulum (3,4). This protein has also been shown to have lyso-platelet-activating factor (PAF) phospholipase C activity (5). A second neutral SMase, nSMase2, also known as SMPD3, is predominantly expressed in the brain (6). The activity of neutral SMases is regulated by oxidative stress, chemotherapeutic drugs, inflammatory cytokines, and apoptotic stimuli (1). nSMase2 has been suggested to regulate extracellular vesicle (EV) biogenesis, release, and uptake in the brain following injury (7,8). Analysis of single and double knockouts of the SMPD2 and SMPD3 has revealed that loss of both genes leads to complete loss of neutral SMase activity, with developmental defects observed with loss of nSMase2 (9,10). Alternate Names FLJ22593; MGC138443; Neutral sphingomyelinase 2; Neutral sphingomyelinase II; NSMA2; nSMase-2; nSMase2; SMPD3; Sphingomyelin phosphodiesterase 3; sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG