CST, 44627S, Chitotriosidase-1/CHIT1 (E4X4I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Chitinase-1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Chitotriosidase-1/CHIT1 (E4X4I) Rabbit Monoclonal Antibody (CST #44627) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Chitotriosidase-1/CHIT1 (E4X4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total Chitotriosidase-1/CHIT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser406 of human Chitotriosidase-1/CHIT1 protein. Background Chitin is an essential biopolymer structural component of many organisms, including arthropods, fungi, and parasites. Chitinases are part of the 18-glycoside hydrolase (18-GH) family of proteins that act to bind and degrade chitin. In mammals, this function provides defense against chitin-containing pathogens. Chitotriosidase-1, also known as Chitinase-1 or CHIT1, is the most abundant of these enzymes. CHIT1 consists of an N-terminal catalytic domain, a C-terminal chitin-binding domain, and a short hinge region that connects the two. CHIT1 activity is implicated in several inflammatory signaling pathways and is considered a sensitive biomarker for activated immune cells. It is produced and secreted by macrophages, microglia, and neutrophils in normal and inflammatory conditions. Elevated levels of CHIT1 in serum is a suggested biomarker for Gaucher's disease, atherosclerosis, pulmonary fibrosis, and some cancers. Elevated CSF levels of CHIT1 is also a suggested biomarker for amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases (1-5). Alternate Names CHI3; CHIT; CHIT1; CHITD; chitinase 1; chitinase 1 (chitotriosidase); Chitinase-1; Chitotriosidase-1; FLJ00314; MGC125322; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG