CST, 4462S, Ran Antibody

Polyclonal Antibody for studying Ran. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ran Antibody (CST #4462) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ran Antibody detects endogenous levels of total Ran protein. This antibody does not cross-react with other small GTPases of the Ras family. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Antibodies are purified by protein A and peptide affinity chromatography. Background Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3). Alternate Names Androgen receptor-associated protein 24; ARA24; Gsp1; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; RAN; RAN, member RAS oncogene family; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein; TC4 Specification REACTIVITY: H M R Hm Mk B SENSITIVITY: Endogenous MW (kDa): 24 SOURCE: Rabbit