CST, 44646S, PXR (E4S1W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PXR. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, PXR (E4S1W) Rabbit Monoclonal Antibody (CST #44646) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity PXR (E4S1W) Rabbit Monoclonal Antibody recognizes endogenous levels of total PXR protein. This antibody detects a 70 kDa band of unknown origin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human PXR protein. Background The pregnane X receptor (PXR) is part of the nuclear hormone receptor superfamily and is a master regulator of xenobiotic metabolism (1). PXR was initially described as being activated by pregnanes and inducing expression of the CYP3A family of steroid hydroxylases (2,3). Similar to PPAR and CAR nuclear receptors, PXR utilizes RXR as a heterodimeric partner and controls target hepatic enzyme genes to control liver metabolism in response to a wide variety of drugs (4). However, PXR is strikingly evolutionarily divergent, and the ligand-binding domain only shares around 75% identity across species. Compounds that readily activate human PXR, such as rifampicin, have little activity in rodents (5,6). In humans, in addition to naturally occurring steroids, PXR is activated by numerous xenobiotics, including anti-cancer drugs paclitaxel and tamoxifen, the HIV protease inhibitor ritonavir, and the anti-diabetic troglitazone (5,7-9). PXR has been implicated in numerous conditions, including inflammatory bowel disease, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD) (10-12). PXR plays a protective role in many tumor types and its expression is generally correlated with better prognosis (13-15). Alternate Names BXR; NR1I2; Nuclear receptor subfamily 1 group I member 2; nuclear receptor subfamily 1, group I, member 2; ONR1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; PAR; PAR1; PAR2; PARq; pregnane X nuclear receptor variant 2; Pregnane X receptor; PRR; PXR; SAR; Steroid and xenobiotic receptor; SXR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG