CST, 44667S, SLC25A37 (F6W1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLC25A37. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SLC25A37 (F6W1R) Rabbit Monoclonal Antibody (CST #44667) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SLC25A37 (F6W1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLC25A37 protein. This antibody detects bands of unknown origin at 60 and 70 kDa in some cell lines by western blot. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp35 of human SLC25A37 protein. Background Mitochondria require iron import for heme and iron-sulfur cluster biogenesis for cellular respiration (reviewed in 1). Perturbation of iron homeostasis can manifest in several pathological conditions. Mitoferrin-1 (SLC25A37) and mitoferrin-2 (SLC25A28) are homologous members of the solute carrier family that are important for iron import into the mitochondria (2). Combinatorial CRISPR screening identified SLC25A37 and the mitochondrial glutathione transporter as important regulators supporting oxidative phosphorylation (3). Mitochondrial depolarization results in a PINK1/Parkin-dependent decrease in SLC25A37 and SLC25A28, and deletion of PINK and Parkin can accelerate K-Ras driven pancreatic tumorigenesis with an increase in mitochondrial iron accumulation (4). Deletion of SLC25A37 homologue in zebrafish inhibited erythroid maturation (5). Decreased expression of SLC25A37 has been described in erythropoietin protoporphyria, in which patients have an accumulation of protoporphyrins in red blood cells (6). Decreased expression of SLC25A37 has also been identified as a risk factor in major depressive disorder (7). Alternate Names HT015; MFRN; MFRN1; Mitochondrial iron transporter 1; Mitochondrial solute carrier protein; mitoferrin; Mitoferrin-1; MSC; MSCP; predicted protein of HQ2217; PRO1278; PRO1584; PRO2217; SLC25A37; solute carrier family 25 (mitochondrial iron transporter), member 37; Solute carrier family 25 member 37; solute carrier family 25, member 37 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG