CST, 44840SF, CasMINI (8H6-A8) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying cas12f bacteria. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, CasMINI (8H6-A8) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #44840) is ready to ship. Product Usage Information This product is the carrier free version of product #53413. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 53413 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CasMINI (8H6-A8) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes transfected levels of CasMINI protein. This antibody may detect several non-specific bands of unknown origin in rat cell lines. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant protein specific to CasMINI protein. Background CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from and ) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a and Class 2 CRISPR endonucleases permit efficient and specific genome editing that may be used as gene therapies for genetic diseases. However, virus-based vehicles used for delivery, like adeno-associated virus (AAV)-based vectors, have a limited packaging capacity. CasMINI was engineered from the naturally occurring type V-F Cas12f (Cas14) system. CasMINI is 529 amino acids, which is significantly smaller than Cpf1/Cas12a and Cas9 proteins (62% and 57% smaller, respectively), making CasMINI more suitable for virus-based packaging for delivery (4). Alternate Names cas14a1; CRISPR-associated endodeoxyribonuclease Cas12f1; Un1Cas12f1 Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 70 Source/Isotype: Mouse IgG1