CST, 63670SF, G4S Linker (E7O2V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Live),ELISA+. Highly specific and rigorously validated in-house, G4S Linker (E7O2V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #63670) is ready to ship. Product Usage Information This product is the carrier free version of product #71645. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 71645 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity G4S Linker (E7O2V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker. Species Reactivity: Human, All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats. Background The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S) or 20-mer (G4S) within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2). Specification REACTIVITY: H All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG