CST, 69463SF, LIMP-2/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SCARB2 mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, LIMP-2/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #69463) is ready to ship. Product Usage Information This product is the carrier free version of product #27960. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 27960 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LIMP-2/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total LIMP-2/SCARB2 protein. LIMP-2/SCARB2 is glycosylated at multiple sites, and it migrates at 80 kDa and 54 kDa in its glycosylated and unglycosylated states, respectively. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly467 of mouse LIMP-2/SCARB2 protein. Background Lysosomal integral membrane protein type 2 (LIMP-2, also known as SCARB2) is a lysosomal membrane glucoprotein encoded by the gene. As a lysosome-enriched protein, LIMP-2/SCARB2 contributes to endosomal and lysosomal transport and function in the cell. β-glucocerebrosidase (GBA), a lysosomal enzyme defective in Gaucher disease and genetically linked to Parkinson's disease, is targeted to lysosomes by binding directly to LIMP-2/SCARB2 via a mannose 6-phosphate-independent pathway (1-3). LIMP-2/SCARB2 may also play a role in the transport of cholesterol from the lysosome to the membrane, an important function as cholesterol contributes to various biophysical properties of cell membranes and may contribute to various diseases (4,5). Interestingly, SCARB2 is upregulated in neurodegenerative diseases, including Lewy body disease (6), and genetic variants in the gene encoding SCARB2 are linked to Parkinson's disease (7), suggesting that altered lysosomal function may contribute to neuropathology in these diseases. LIMP-2/SCARB2 also serves as a receptor for enterovirus 71 and coxsackieviruses (8). Alternate Names 85 kDa lysosomal membrane sialoglycoprotein; 9330185J12Rik; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2; Cd36l; Cd36l2; LGP; LGP85; LIM; LIMP II; LIMP-2; Lysosome membrane protein 2; Lysosome membrane protein II; MLGP85; Scarb2; Scavenger receptor class B member 2; scavenger receptor class B, member 2; SCRB2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 54, 80 Source/Isotype: Rabbit IgG