CST, 72607T, Integrin beta2 (E9O7W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Integrin Beta2 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin beta2 (E9O7W) Rabbit Monoclonal Antibody (CST #72607) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 49893 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Integrin beta2 (E9O7W) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse Integrin β2 protein. This antibody does not cross-react with human Integrin β2 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser84 of mouse Integrin β2 protein. Background Integrins are transmembrane glycoproteins that form heterodimers consisting of one α and one β subunit. Integrin dimers act as receptors for extracellular matrix proteins and cell-surface ligands. Integrin signaling to (inside-out) and from (outside-in) extracellular molecules regulates multiple cellular processes, such as development, wound healing, immune response, invasion, metastasis, and angiogenesis (1,2). Integrin β2 (CD18) is the β subunit of the leukocyte-specific integrin family. Leukocyte integrins include Integrin β2 (CD18)/αL (CD11a) (LFA-1, lymphocyte function associated antigen 1), Integrin β2 (CD18)/αM (CD11b) (Mac-1), Integrin β2 (CD18)/αX (CD11c), and Integrin β2 (CD18)/αD (CD11d). These integrins bind to immunoglobulin superfamily members, such as ICAM-1 and VCAM-1 on endothelial cells, to mediate firm adhesion and transendothelial migration of leukocytes (3). Integrin β2 (CD18) deficiency results in LAD (leukocyte adhesion deficiency), a disease characterized by impairment of leukocyte recruitment, resulting in an inability to fight infection (4). Alternate Names 2E6; AI528527; Cd18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; integrin beta 2; Integrin beta-2; ITB2; Itgb2; LAD; LCAMB; Lfa1; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta; MF17; OTTMUSP00000021575 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG